cid e7635ee603e5865fd4882f755076d7faf84930f65ed3497bcb0685aa21f6051e · original · open access · via openalex
Title: High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation Authors: Abigail L. Clutterbuck; Julia R. Smith; David Allaway; Pat Harris; Susan Liddell; Ali Mobasheri Venue: Journal of Proteomics Year: 2011 Canonical: doi:10.1016/j.jprot.2011.02.017 URL: https://doi.org/10.1016/j.jprot.2011.02.017 Collector: openalex OA: true ABSTRACT This study employed a targeted high-throughput proteomic approach to identify the major proteins present in the secretome of articular cartilage. Explants from equine metacarpophalangeal joints were incubated alone or with interleukin-1beta (IL-1β, 10ng/ml), with or without carprofen, a non-steroidal anti-inflammatory drug, for six days. After tryptic digestion of culture medium supernatants, resulting peptides were separated by HPLC and detected in a Bruker amaZon ion trap instrument. The five most abundant peptides in each MS scan were fragmented and the fragmentation patterns compared to mammalian entries in the Swiss-Prot database, using the Mascot search engine. Tryptic peptides originating from aggrecan core protein, cartilage oligomeric matrix protein (COMP), fibronectin, fibromodulin, thrombospondin-1 (TSP-1), clusterin (CLU), cartilage intermediate layer protein-1 (CILP-1), chondroadherin (CHAD) and matrix metalloproteinases MMP-1 and MMP-3 were detected. Quantitative western blotting confirmed the presence of CILP-1, CLU, MMP-1, MMP-3 and TSP-1. Treatment with IL-1β increased MMP-1, MMP-3 and TSP-1 and decreased the CLU precursor but did not affect CILP-1 and CLU levels. Many of the proteins identified have well-established extracellular matrix functions and are involved in early repair/stress responses in cartilage. This high throughput approach may be used to study the changes that occur in the early stages of osteoarthritis.