cid 0314aed0c27d5ce1571162288499ef8d8ac20c71826a2af27724e25fe00803d7 · original · open access · via openalex
Title: Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach Authors: Mayya P. Razgonova; Yulia N. Zinchenko; Darya Konstantinovna Kozak; V.A. Kuznetsova; Alexander M. Zakharenko; Sezai Erċışlı; Kirill Sergeevich Golokhvast Venue: Molecules Year: 2022 Canonical: doi:10.3390/molecules27238228 URL: https://doi.org/10.3390/molecules27238228 Collector: openalex OA: true ABSTRACT In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon. High-performance liquid chromatography (HPLC) was used in combination with an amaZon SL BRUKER DALTONIKS ion trap (tandem mass spectrometry) to identify target analytes in soybean extracts. The results of initial studies revealed the presence of 63 compounds, and 45 of the target analytes were identified as polyphenolic compounds.